Invitrogen MCP-1 Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen MCP-1 Human ELISA Kit, 10 x 96 Tests
The Human Monocyte Chemoattractant Protein-1(Hu MCP-1) ELISA quantitates Hu MCP-1 in human serum, plasma,amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MCP-1.
Principle of the method
The Human MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 8.7%
- Intraassay CV: 4.7%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- HRP-conjugated Detection Antibody
- Wash Buffer
- Chromogen
- Stop Solution
- Controls
- Adhesive Plate Covers
There are no specifications
There are no report
You May Also Like
Invitrogen IL-17A (homodimer) Mouse Uncoated ELISA Kit, 20 x 96 Tests
$ On demand