Invitrogen™ MessageAmp™ Premier RNA Amplification Kit, 10 Reactions

Invitrogen™ MessageAmp™ Premier RNA Amplification Kit, 10 Reactions

The MessageAmp™ Premier RNA Amplification Kit is the latest innovation for the preparation of RNA samples for microarray analysis. The kit builds upon the linear RNA amplification method and reagents developed for MessageAmp™ II Kits with enhancements that create a simplified workflow, improved performance, and more flexible RNA input requirements. All MessageAmp™ kits employ the proven methodology for RNA sample preparation and labeling based on the original T7 in vitro transcription (IVT) amplification technology, known as the Eberwine or reverse transcription-IVT (RT-IVT) method. The RT-IVT method is considered the gold standard for sample preparation in microarray-based expression profiling. 

Features

• Lowest RNA input requirements for a single round of amplification: 20 ng total RNA yields enough biotin-modified aRNA for most microarray formats, with results equivalent to those from samples prepared from significantly more starting material using other RT-IVT kits
• Very flexible RNA input requirements: 20–500 ng total RNA, depending on the tissue or cell type
• Streamlined workflow: depending on the RNA input amounts, only a single day of processing time is required to complete a microarray experiment
• Simplified protocol and streamlined reagents
• Complete kit: everything needed to prepare biotin-modified aRNA is included
• Rigorous quality control that includes GeneChip® analysis
• T7 MEGAscript® IVT delivers up to 50,000-fold amplification
• Single-tube format
• Easy-to-use, high-recovery, magnetic-bead aRNA purification

Contents

• First Strand Enzyme Mix (-20°C): 11 µl 
• First Strand Buffer Mix (-20°C): 44 µl 
• Second Strand Enzyme Mix (-20°C): 22 µl 
• Second Strand Buffer Mix (-20°C): 55 µl 
• T7 Enzyme Mix (-20°C): 66 µl 
• T7 Biotin IVT Mix (-20°C): 220 µl 
• Control RNA 1 mg/ml (-20°C): 10 µl 
• Nuclease-free Water (any temperature): 1.75 ml 
• aRNA Binding Buffer Concentrate (room temperature): 600 µl 
• RNA Binding Beads (4°C): 120 µl 
• aRNA Wash Solution Concentrate (room temperature): 10 ml 
• aRNA Elution Solution (4°C or room temperature): 5 ml 
• 5X Array Fragmentation Buffer (room temperature): 1 ml 
• 8-Strip PCR Tubes & Caps (room temperature): 10 Strips
• U-Bottom Plates (room temperature): 1 Plate
• Reservoir (room temperature): 1 Reservoire
• Nuclease-free Water (any temperature): 10 mL