Invitrogen MIP-3 alpha/CCL20 Cell Lysate Human ELISA Kit, 5 x 96 Tests

Invitrogen MIP-3 alpha/CCL20 Cell Lysate Human ELISA Kit, 5 x 96 Tests

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α.

Principle of the method

The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Human MIP-3a Antibody Coated wells, 96-well plate, 1 plate 
• Human MIP-3a Biotin Conjugate, 2 vials 
• Human MIP-3a Standard, recombinant human MIP-3a, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL 
• Sample Diluent Buffer (5X), 10 mL 
• Assay Diluent (5X), 15 mL
• Cell lysate buffer (2X), 5 mL 
• Streptavidin-HRP (300X), 0.2 mL 
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2