Invitrogen MUCIN 16/CA125 Human ELISA Kit, 10 x 96 Tests

Invitrogen MUCIN 16/CA125 Human ELISA Kit, 10 x 96 Tests

The Human MUCIN 16/CA125 ELISA quantitates Hu Mucin 16 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Mucinn 16.

Principle of the method

The Human Mucin 16 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Human Mucin 16 Antibody Coated wells, 96-well plate, 10 plate 
• Human Mucin 16 Biotin Conjugate, 20 vials 
• Human Mucin 16 Standard, recombinant human mucin 16, 20 vials 
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent (5X), 10 x 15 mL 
• Streptavidin-HRP (800X), 10 x 0.2 mL 
• TMB Substrate, 10 x 12 mL 
• Stop Solution, 10 x 8 mL 
• Adhesive Plate Covers, 20