Invitrogen NAP-2/PPBP Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen NAP-2/PPBP Human ELISA Kit, 10 x 96 Tests
The Human NAP-2/PPBP ELISA quantitates Hu NAP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NAP-2.
Principle of the method
The Human NAP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Storage: 2-8°C
Contents
- Human NAP-2 Antibody Coated wells, 96-well plate, 1 plate
- Human NAP-2 Biotin Conjugate, 2 vials
- Human NAP-2 Standard, recombinant human NAP-2, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (200X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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