Invitrogen™ No-Stain™ Protein Labeling Reagent, 10 Reactions

Invitrogen™ No-Stain™ Protein Labeling Reagent, 10 Reactions

The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any commonly available imager. No-Stain reagent does not require any particular gels or other reagents and is compatible with gel stains and western workflows.

Features

• Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 µg)
• Sensitive—lower limit of detection of 20 ng per band
• Specific—forms bonds only with the lysine side chains of proteins. Unbound reagent does not emit, thereby enabling a superior signal-to-noise ratio
• Flexible—no need to change your gels to get stain-free convenience. No-Stain reagent provides stain-free convenience with any gel type (precast or pour-your-own gel)

Contents

• No-Stain activator: 200 µL
• No-Stain derivatizer: 200 µL
• 2 x No-Stain labeling buffer: 5 mL