Invitrogen™ Novex™ pH 3-10 IEF Protein Gels, 1.0 mm, 12-well
On demand
Order number :
EC66552BOX
Invitrogen™ Novex™ pH 3-10 IEF Protein Gels, 1.0 mm, 12-well
Novex® pH 3–10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.
IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.
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