Invitrogen™ Oregon Green™ 488 BAPTA-1 dextran, Potassium Salt, 10,000 MW, Anionic
On demandInvitrogen™ Oregon Green™ 488 BAPTA-1 dextran, Potassium Salt, 10,000 MW, Anionic
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated indicators using patch pipette, microinjection, or our Influx pinocyte-loading reagent. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms
Features
Calcium Indicator (Cell-Impermeant Salts, Dextran Conjugates) Specifications:
- Label (Ex/Em): Oregon Green™ 488 BAPTA-1 (494/523 nm)
- Fluorescence intensity increase upon binding Ca2+: ∼14 fold
- Dextran size: 10,000 MW
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader