Invitrogen™ OxyBURST™ Green H2DCFDA, SE
On demand
Order number :
D2935
Invitrogen™ OxyBURST™ Green H2DCFDA, SE
OxyBURST™ Green H2DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST™ Green H2DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST™ Green H2DCFDA conjugates are nonfluorescent until they are oxidized to the corresponding fluorescein derivatives
Features
- Product is air sensitive and should be stored under dry argon or nitrogen
- Product may be dissolved in DMSO, DMF, or ethanol for use
- Indicator is cell permeant (cell loading protocols are available in the literature)
- Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
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