Invitrogen PAI-1 Monkey ELISA Kit, 10 x 96 Tests
On demandInvitrogen PAI-1 Monkey ELISA Kit, 10 x 96 Tests
The Monkey PAI-1 (SERPINE1) ELISA quantitates Rhesus Mk PAI-1 in monkey serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk PAI-1.
Principle of the method
The Monkey PAI-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Rhesus Monkey PAI-1 Antibody Coated wells, 96-well plate, 1 plate
- Rhesus Monkey PAI-1 Biotin Conjugate, 2 vials
- Rhesus Monkey PAI-1 Standard, recombinant rhesus monkey PAI-1, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent (5X), 15 mL
- Streptavidin-HRP (250X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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