Invitrogen PAI1 Human ELISA Kit, 96 Tests, 100 µL

Invitrogen PAI1 Human ELISA Kit, 96 Tests, Sample volume 100 µL

The Human Plasminogen Activator Inhibitor-1 (Hu PAI-1) ELISA quantitates Hu PAI-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant HuPAI-1.

Principle of the method

The Human PAI-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 7.7%
• Intraassay CV: 4.3%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Antibody Coated Plate; 96 well plate, 1 plate
• Hu PAI-1 Biotin Conjugate (biotin-labeled anti-PAI-1). Contains 0.1% sodium azide. 11 mL
• Hu PAI-1 Standard. Lyophilized, recombinant Hu PAI-1. Contains 0.1% sodium azide. 2 vials
• Wash Buffer Concentrate (25X). 100mL
• Standard Diluent Buffer. Contains 0.1% sodium azide. 25 mL
• Streptavidin-HRP (100X). 0.15 mL
• Streptavidin HRP Diluent. 25 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB). 25 mL
• Stop Solution. 25 mL
• Adhesive Plate Covers. 4