Invitrogen PAPP-A Human ELISA Kit, 96 Tests

Invitrogen PAPP-A Human ELISA Kit, 96 Tests

The Human PAPP-A ELISA quantitates Hu PAPP-A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PAPP-A.
Principle of the method
The Human PAPP-A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Human PAPP-A Antibody Coated wells, 96-well plate, 1 plate
• Human PAPP-A Biotin Conjugate, 2 vials
• Human PAPP-A Standard, recombinant human PAPP-A, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent D (5X), 15 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (500X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2