Invitrogen™ pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
On demandInvitrogen™ pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells. pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments
Features
- pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
- Excitation/emission of 560/585 nm
- Excellent photostability
- Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488
- For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Instrument
There are no specifications
There are no report
You May Also Like
Thermo Scientific™ RXRB Recombinant Human Protein, Ligand Binding Domain, GST-Tagged
$ 2,323
On demand
Invitrogen™ Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor™ 488 Conjugate
$ 345
On demand
Invitrogen™ Lectin GS-II From Griffonia simplicifolia, Alexa Fluor™ 647 Conjugate
$ 327