Invitrogen Pierce ERK1/2 Near Infrared In-Cell ELISA Kit, 96 Tests

Invitrogen Pierce ERK1/2 Near Infrared In-Cell ELISA Kit, 96 Tests 

The ERK1/2 Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells.

Principle of the method

This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.

Features

• Homogenous (no wash): No
• Storage: 2-8°C

Contents

• Blocking Buffer, 50mL
• 20X Tris Buffered Saline, 50mL
• Surfact-Amps® 20 (10% Tween®-20 Detergent), 10mL
• Surfact-Amps X-100 (10% Triton® X-100 Detergent), 10mL
• DyLight® 680 Goat Anti-Mouse IgG (H+L), 55μL
• DyLight 800 Goat Anti-Rabbit IgG (H+L), 55μL
• Thin Plate Seal Assembly, 8 each
• Anti-ERK 1 & 2 (T 202/Tyr 204) Antibody
• Anti-ERK1/2 Antibody