Invitrogen™ Platinum™ Tfi Exo(-) DNA Polymerase

Invitrogen™ Platinum™ Tfi Exo(-) DNA Polymerase

Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

Contents

• Platinum™ Tfi DNA Polymerase (500 units total, at 5U/µL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL).