Invitrogen™ Qdot™ Streptavidin Sampler Kit

Invitrogen™ Qdot™ Streptavidin Sampler Kit

Qdot® streptavidin conjugates consist of a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Each of the six conjugates in the Qdot® Streptavidin Sampler Kit is supplied as a 1 µM solution (50 µL volume).

Features

• Streptavidin Qdot® conjugates have emission maxima of ∼525, ∼565, ∼585, ∼605, ∼655, and ∼705 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more