Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits, 500 Assays

Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits

The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining the protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures using a Qubit 4 Fluorometer. This protein kit can deliver accurate protein quantitation across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods. The assay is compatible with many common contaminants, such as detergents, reducing reagents (e.g., DTT, β-mercaptoethanol), salts, and nucleic acids.

For use witn equipment: Qubit 4 Fluorometer

Features

• Broad dynamic range— 0.1–20 mg/mL, measure samples without dilution
• Two-point standard curve— eliminate time-consuming standard preparation
• Automatic concentration determination— receive protein concentration instantaneously; no need for offline calculations
• Compatible— accurate in the presence of reducing reagents, detergents, and other common buffer components

Contents

• The Qubit Protein BR Assay Kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert the sample into a Qubit 4 instrument and read the concentration.