Invitrogen RANTES Feline ELISA Kit, 5 x 96 Tests

Invitrogen RANTES Feline ELISA Kit, 5 x 96 Tests

The Feline RANTES/CCL5 ELISA quantitates Cat RANTES in feline serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Cat RANTES.

Principle of the method

The Feline RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Storage: 2-8°C

Contents

• Feline RANTES Antibody Coated wells, 96-well plate, 1 plate 
• Feline RANTES Biotin Conjugate, 2 vials 
• Feline RANTES Standard, recombinant feline RANTES, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL 
• Streptavidin-HRP (200X), 0.2 mL
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2