Invitrogen™ Resistin Human ELISA Kit
On demandInvitrogen™ Resistin Human ELISA Kit
The Human Resistin ELISA quantitates Hu Resistin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Resistin.
Principle of the method
The Human Resistin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 5.6%
- Intraassay CV: 3.7%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu Resistin Standard, lyophilized, 1 vial
- ELISA Buffer (10X), 2 × 30 mL
- Antibody Coated Wells, 96-well plate, 1 plate
- Detection Antibody, 30 μL
- HRP labeled Streptavidin, lyophilized, 2 μg
- Wash Buffer Concentrate (10X), 2 × 30 mL
- TMB Substrate Solution, 12 mL
- Stop Solution, 12 mL
- Adhesive Plate Covers, 2
There are no specifications
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