Invitrogen™ RNase T1 (1 U/μL)

Invitrogen™ RNase T1 (1 U/μL)

Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µL (1U/ µL). RNase T1 can be used to perform boundary experiments to define the minimal RNA sequence required for selectable activities such as protein binding or catalysis. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.

Contents

• RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation⁄Inactivation Buffer, and Gel Loading Buffer II should all be stored at –20°C.