Invitrogen SDF-1 alpha/CXCL12 Mouse ELISA Kit, 5 x 96 Tests

Invitrogen SDF-1 alpha/CXCL12 Mouse ELISA Kit, 5 x 96 Tests

The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α.

Principle of the method

The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%    
• Shipping condition: Wet or Dry Ice    
• Storage: 2-8°C

Contents    

• Mouse SDF-1 alpha Antibody Coated wells, 96-well plate, 1 plate 
• Mouse SDF-1 alpha Biotin Conjugate, 2 vials 
• Mouse SDF-1 alpha Standard, recombinant mouse SDF- 1 alpha, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (200X), 0.2 mL 
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2