Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR

The SuperScript™ First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 µg of total RNA. After synthesis, the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations. In conjunction with PCR, the system can be used to detect the presence of rare messages, to quantitate the amount of specific mRNA from small numbers of cells, or to clone specific cDNAs without constructing an entire cDNA library. The system is flexible, allowing the use of any PCR enzyme. Combine with AccuPrime™ Taq DNA Polymerase or Platinum™ Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications.

Contents

• Oligo(dT)12-18 (0.5 µg/µl): 50 µl 
• Random hexamers (50 ng/µl): 250 µl 
• 10X RT buffer: 1 ml 
• 25 mM Magnesium Chloride: 500 µl 
• 0.1 M DTT: 250 µl 
• 10 mM dNTP mix: 250 µl 
• SuperScript™ II RT (50 U/µl): 50 µl 
• RNaseOUT™ (40 U/µl): 100 µl 
• E. coli RNase H (2 U/µl): 50 µl 
• DEPC-treated water: 1.2 ml 
• Control RNA (50 ng/µl): 15 µl 
• Control Primer A (10 µM): 20 µl 
• Control Primer B (10 µM): 20 µl