Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR, 50 Reactions

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR, 50 Reactions

SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript™ III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). The simple, time-saving reaction set-up uses just two tubes: a 2X Reaction Mix and an Enzyme Mix.

Contents

SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR is supplied with:

• 2X RT Reaction Mix (500 µL, includes oligo(dT)20, random hexamers, MgCl2, and dNTPs)
• RT Enzyme Mix (100 µL, includes SuperScript™ III RT and RNaseOUT™ Recombinant Ribonuclease Inhibitor)
• E. coli RNase (50 µL)