Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, 25 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
25 Reactions
Order number :
12574018
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, 25 Reactions
The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA.
Features
- Sensitive—routine detection down to 0.01 pg total RNA
- Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
- Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
- Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
- Built-In Hot Start
Contents
- 50 µL SuperScript™ III RT/Platinum™ Taq Mix
- 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 3.2 mM MgSO4)
- 500 µL 5 mM Magnesium Sulfate
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