Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, 25 Reactions

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, 25 Reactions

The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA.

Features

• Sensitive—routine detection down to 0.01 pg total RNA
• Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
• Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
• Built-In Hot Start

Contents

• 50 µL SuperScript™ III RT/Platinum™ Taq Mix
• 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 3.2 mM MgSO4)
• 500 µL 5 mM Magnesium Sulfate