Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase, 25 Reactions

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase, 25 Reactions

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR. This system consists of two major components: SuperScript III RT/ Platinum Taq High Fidelity Enzyme Mix and 2X Reaction Mix. The enzyme mix combines SuperScript III Reverse Transcriptase and Platinum Taq DNA Polymerase High Fidelity, which is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps. The system can detect a wide range of RNA targets (up to 10 kb in length) at variable concentrations (1 pg to 1 µg of total RNA).

Contents

• 25µL SuperScript™ III RT/Platinum™ Taq High Fidelity Enzyme Mix
• 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 2.4 mM MgSO₄)
• 500 µL 5 mM Magnesium Sulfate