Invitrogen™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit, 100 Reactions

Invitrogen™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit, 100 Reactions

The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection

For use with: 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ

Features

• SuperScript™ III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes for superior detection performance
• SYBR™ Green I dye for easy and convenient detection
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

Contents

• SuperScript™ III RT/Platinum™ Taq Mix: 100 µl
• 2X SYBR™ Green Reaction Mix: 2 x 1.25 ml
• Magnesium Sulfate (50 mM): 1 ml
• ROX Reference Dye (25 µM): 100 µl
• 20X Bovine Serum Albumin (1 mg/ml): 300 µl