Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit, 50 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
50 Reactions
Order number :
11750150
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
Features
- Compatible with a wide range of mammalian cell types grown under different treatment conditions
- Single-tube format minimizes reagent loss, sample loss, and handling time
- Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
- High-quality cDNA generated for use in a variety of applications, including PCR and qPCR
- Simple protocol takes around 37 minutes
- Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers
- Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA
Contents
- SuperScript IV CellsDirect Lysis Solution, 1.25 mL
- SuperScript IV CellsDirect Stop Solution, 250 µL
- Lysis Enhancer, 25 µL
- Dnase I, 55 µL
- SuperScript IV RT Master Mix, 400 µL
- SuperScript IV No RT Control, 400 µL
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