Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit, 192 Reactions

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit, 192 Reactions

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng). It contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format. A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy. Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours. The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR.

Features

• Superior sensitivity—easy detection of low abundant targets from as low as single cell or 2 pg of total RNA
• High quality cDNA—full-length transcript information with uniform coverage
• Streamlined workflow—simple one-tube protocol
• Time saving—short lysis and reverse transcription times
• Universal—global preamplification compatible with downstream analysis by NGS or real-time PCR

Contents

Four packs, each containing: 

• 10X Lysis Buffer, 48 μL
• RNase Inhibitor, 19 μL
• Capturing Oligonucleotide, 48 μL
• 4X SuperScript IV cDNA Synthesis Master Mix, 240 μL
• Template Switching Oligonucleotide, 48 μL
• 2X Platinum SuperFi U Preamplification Master Mix, 2 x 1.2 mL
• Preamplification primer, 48 μL
• Water, nuclease-free, 2 x 1.25 mL