Invitrogen™ T4 DNA Ligase (5 U/µL)
On demand
Order number :
15224041
Invitrogen™ T4 DNA Ligase (5 U/µL)
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is available.
Contents
- T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6)
- 50 mM MgCl2
- 5 mM ATP
- 5 mM DTT
- 25% (w/v) polyethylene glycol-8000]
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