Invitrogen™ T4 DNA Polymerase, 50 Unites
On demandInvitrogen™ T4 DNA Polymerase, 50 Unites
T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available.
Applications: Labeling double-stranded linear DNA by replacement synthesis. Oligonucleotide-directed, site-specific mutagenesis. 3´ end-labeling of double-stranded DNA. Polishing both 5´ or 3 overhangs to make blunt ends.
Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid.
Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template•primer.
Contents
- T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.
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