Invitrogen™ T7 RNA Polymerase, 2500 Units
On demand
Order number :
18033019
Invitrogen™ T7 RNA Polymerase, 2500 Units
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.
Application: Synthesis of labeled and unlabeled RNA transcripts (1).
Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid.
Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a
transcription reaction.
Contents
- T7 RNA Polymerase (50 U/µL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2 , 10 mM spermidine-(HCl)3 , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
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