Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells, 40 Reactions
On demand
Order number :
K207040
Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells, 40 Reactions
The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
Features
- Fast & convenient—15-minute, room-temperature ligation
- Efficient—blue/white screening and >80% clones with correct insert
- Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free—eliminates any enzymatic modifications of the PCR product
- Streamlined—does not require the use of PCR primers that contain restriction sites
Contents
- Linearized pCR™II vector
- ExpressLink™ T4 DNA ligase
- 5X ExpressLink™ T4 DNA ligation buffer
- dNTPs
- 10X PCR buffer
- Sterile water
- Controls
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