Invitrogen TACE/ADAM17 Human ELISA Kit, 96 Tests

Invitrogen TACE/ADAM17 Human ELISA Kit, 96 Tests

The Human TACE/ADAM17 ELISA quantitates Hu TACE in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TACE.

Principle of the method

The Human TACE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: <12%
• Intraassay CV: <10%
• Homogenous (no wash): No
• Storage: 2-8°C

Contents

• Human TACE Antibody Coated wells, 96-well plate, 1 plate
• Human TACE Biotin Conjugate, 2 vials
• Human TACE Standard, recombinant human TACE, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (200X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2