Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit, 100 Reactions

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit, 100 Reactions

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. TaqMan Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Fast Advanced reverse transcription enzyme mix, 2x RT buffer, and best-in-class TaqMan Fast Advanced master mix. The TaqMan Fast Advanced Cells-to-Ct Kit gives you all the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 80 minutes.

Features

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new TaqMan Fast Advanced Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Contents

• 20X Fast Advanced RT Enzyme Mix: 250 µL 
• 2X Fast Advanced RT Buffer: 5 mL 
• Lysis Solution: 5 mL 
• Stop Solution: 550 µL 
• DNase I: 55 µL 
• TaqMan Fast Advanced Master Mix: 5 mL