Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit, 100 Reactions

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit, 100 Reactions

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification. This kit saves time and offers a simple workflow that is suitable for a few samples or can be easily incorporated into automated, high-throughput applications.

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan™ Gene Expression Cells-to-CT™ Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan™ Gene Expression Master Mix for real-time PCR analysis. TaqMan™ primer⁄probe sets are sold separately.

For use with equipments: 7000 System, 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system

Features

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TaqMan™ Gene Expression Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Contents

• Stop Solution: 500 µl tubes 
• DNase I: 55 µl tube 
• 20X RT Enzyme Mix: 275 µL tube 
• Lysis Solution: 5.5 mL tube
• 2X RT Buffer: 5.5 mL tube
• TaqMan™ Gene Expression Master Mix: 5 mL tube