Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit

The Ambion™ TaqMan PreAmp Cells-to-C_T Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification. The protocol includes an intermediate amplification step (pre-amplification) between reverse transcription and real-time PCR in which cDNA is enriched for up to 100 gene targets of the user’s choice without amplification bias.

For use with equipments: 7000 System, 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system

Features

• Complete, validated solution—Optimized workflow includes cell lysis reagents, DNase, RT reagents, PreAmp reagents, and TaqMan™ Gene Expression Master Mix
• Fast, simple and convenient—Prepare samples in 7 minutes; eliminate tedious RNA isolation
• Extend limited samples—Increase real-time PCR potential by up to 64-fold; study up to 100 genes at once
• Accurate results—Uniform amplification without introducing bias
• Robust performance—Linear detection from 10 to 100,000 cells; results equivalent to purified RNA

Contents

• Sample prep rxns: 40 Reactions
• Dnase rxns: 40 Reactions
• Reverse transcription rxns: 40 Reactions
• 20 µL PCR rxns: 100 Reactions