Invitrogen TGF beta-2 Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen TGF beta-2 Human ELISA Kit, 10 x 96 Tests
The Human Transforming Growth Factor beta2 (TGFβ2) ELISA quantitates Hu TGFβ2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβ2.
Principle of the method
The Human TGFβ2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
Contents
- Human TGFB2 Antibody Coated wells, 96-well plate, 10 plate
- Human TGFB2 Biotin Conjugate, 20 vials
- Human TGFB2 Standard, recombinant human TGFB2, 20 vials
- Wash Buffer Concentrate (20X), 12 x 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 10 x 30 mL
- Assay Diluent B (5X), 10 x 15 mL
- Streptavidin-HRP (500X), 10 x 0.2 mL
- TMB Substrate, 10 x 12 mL
- Stop Solution, 10 x 8 mL
- Adhesive Plate Covers, 20
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