Invitrogen™ Thiol Fluorescent Detection Kit

Invitrogen™ Thiol Fluorescent Detection Kit

The Protein Thiol Detection research-use-only kit is a fluorescent assay designed for the quantification and detection of protein thiol in common biological buffers including biological buffers including Tris, phosphate, and citrate buffers.

This complete, ready-to-use kit includes black 96-well plate(s), N-acetylcysteine standard, detection reagent, assay buffer, and other components to perform the assay. A 96-well microplate reader capable of reading fluorescent emission at 510 nm, with excitation at 390 nm, is required for use of this kit.

Features

• Sample types: common biological buffers including Tris, phosphate, and citrate buffers
• Sensitivity: 4.62 nM
• Standard curve range: 78 nM–10,000 nM
• Reactivity: species independent
• Gene ID (Entrez): Non-Gene
• Gene Symbol: Non-Gene
• Storage: Store at –20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Target Kit Named: Thiol
• For Use With (Equipment): Microplate Reader
• Immunoassay Kit Format: Detection Kit

Content

• N-Acetylcysteine Standard; 100,000 nM N-acetyl-L-cysteine in a special stabilizing solution, 220 μL
• Black Microtiter Plate, 1 plate
• Detection Reagent; r econstitute with Dry DMSO, 2 vials
• Dry DMSO (Dimethyl sulfoxide), 4 mL
• Assay Buffer Concentrate (2X), 60 mL
• Plate Sealer, 1