Invitrogen TNF alpha Canine ELISA Kit, 10 x 96 Tests

Invitrogen TNF alpha Canine ELISA Kit, 10 x 96 Tests

The Canine Tumor Necrosis Factor alpha(Dog TNFα) ELISA quantitates Dog TNFα in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog TNFα.
Principle of the method
The Canine TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Canine TNF-alpha Antibody Coated wells, 96-well plate, 10 plate
• Canine TNF-alpha Biotin Conjugate, 20 vials
• Canine TNF-alpha Standard, recombinant canine TNF-alpha, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 10 x 30 mL
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (350X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20