Invitrogen TNF alpha Human ELISA Kit, 100 µL, 96 Tests

Invitrogen TNF alpha Human ELISA Kit, Sample Volume 100 µL, 96 Tests

The Human Tumor Necrosis Factor alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα.
Principle of the method
The Human TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 7.5%
• Intraassay CV: 4.4%

Contents    

• Hu TNF‑a Standard, lyophilized; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 8 mM sodium azide, 25 mL
• Incubation Buffer; contains 8 mM sodium azide, 11 mL
• Antibody‑Coated Wells, 96-well plate, 1 plate
• Hu TNF‑a Biotin Conjugate; contains 8 mM sodium azide, 11 mL
• Streptavidin‑HRP, (100X ); contains 1.3 mM thymol, 0.125 mL
• Streptavidin‑HRP Diluent; contains 1.3 mM thymol and 0.05% Proclin™ 300, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3