Invitrogen TNF alpha Human ELISA Kit, 200 µL, 96 Tests

Invitrogen TNF alpha Human ELISA Kit, Sample Volume 200 µL, 96 Tests

The Human Tumor Necrosis Factor alpha (Hu TNF-α) ELISA quantitates Hu TNF-α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNF-α.
Principle of the method
The Human TNF-α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 6%
• Intraassay CV: 3%

Contents    

• Incubation Buffer (phosphate buffer), with bovine serum albumin and thymol), 6 mL
• Zero Calibrator; in human serum, with benzamidine and thymol; lyophilized (see vial label for quantity and reconstitution volume), 2 vial
• Calibrators 1 to 5; in human serum, with benzamidine and thymol; lyophilized (see vial label for quantity and reconstitution volume), 5 vials
• Controls 1 and 2; in human serum, with benzamidine and thymol; lyophilized. Refer to vial label for reconstitution volume and range, 2 vials
• TNF-α Antibody-Coated Wells, 96-well strip-well plate, 1 plate
• Anti-TNF-α-HRP Conjugate; in Tris-Maleate buffer with bovine serum albumin and thymol, 0.75 mL
• Conjugate Buffer; in Tris-Maleate buffer with bovine serum albumin, EDTA, and thymol, 6 mL
• Wash Solution Concentrate (200X), 10 mL
• Chromogenic TMB (tetramethylbenzidine) in DMF, 12 mL
• Stop Solution (1 N HCl), 12 mL