Invitrogen TNF alpha Human Matched Antibody Pair, 10 x 96 Tests
On demandInvitrogen TNF alpha Human Matched Antibody Pair, 10 x 96 Tests
The Human TNF-alpha Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TNF-alpha. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
Contents
- Capture (coating) Antibody
- Detection Antibody
- SAV-HRP conjugate
- Standard
- Sample Diluent
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