Invitrogen TNF alpha Porcine ELISA Kit, 96 Tests

Invitrogen TNF alpha Porcine ELISA Kit, 96 Tests

The Porcine Tumor Necrosis Factor alpha (Por TNFα) ELISA quantitates Por TNFα in porcine serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por TNFα1.
Principle of the method
The Pociner TNFαsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 7.3%
• Intraassay CV: 6%

Contents    

• Sw TNF-α Standard; lyophilized. 2 vials
• Standard Diluent Buffer; contains 8 mM sodium azide, 25 mL
• Incubation Buffer; contains 8 mM sodium azide, 11 mL
• Antibody-Coated Wells, 96-well plate, 1 plate
• Sw TNF-α Biotin Conjugate (100X concentrate); contains 8 mM sodium azide, 0.125 mL
• Sw TNF-α Biotin Conjugate Diluent Buffer; contains 8 mM sodium azide, 12.5 mL
• Streptavidin-Peroxidase (HRP) (100X), 0.125 mL
• Streptavidin-Peroxidase (HRP) Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 4