Invitrogen TNF alpha Rat ELISA Kit, Sen. <4 pg/mL, 2 x 96 Tests

Invitrogen TNF alpha Rat ELISA Kit, Sen. <4 pg/mL, 2 x 96 Tests

The Rat Tumor Necrosis Factor alpha(Rt TNFα) ELISA quantitates Rt TNFα in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat TNFα.
Principle of the method
The Rat TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 8.3%
• Intraassay CV: 5.8%

Contents    

• Rt TNF-α Standard, lyophilized; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Wells, 96-well plate, 1 plate
• Rt TNF-α Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Incubation Buffer, 12 mL
• Streptavidin-HRP (100X), 0.15 mL
• Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 3