Invitrogen™ TransFluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm (488/560), 2% Solids

Invitrogen™ TransFluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm (488/560), 2% Solids

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ TransFluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with two (or more) fluorescent dyes to produce a microspheres that can be excited by the 488 nm or the 514 nm spectral lines of the argon-ion laser but emit at much longer wavelengths, facilitating multicolor experiments and allowing the use of broadband excitation and emission filters. TransFluoSpheres™ microspheres typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Key Applications of Microspheres

• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)