Invitrogen VEGF Receptor 2/KDR Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen VEGF Receptor 2/KDR Human ELISA Kit, 10 x 96 Tests
The Human Vascular Endothelial Growth Factor Receptor 2/KDR (Hu VEGFR2) ELISA quantitates Hu VEGFR2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR2.
Principle of the method
The Human VEGFR2 solid-phase
sandwich ELISA (enzyme-linked immunosorbent assay) is designed to
measure the amount of the target bound between a matched antibody pair. A
target-specific antibody has been pre-coated in the wells of the
supplied microplate. Samples, standards, or controls are then added into
these wells and bind to the immobilized (capture) antibody. The
sandwich is formed by the addition of the second (detector) antibody, a
substrate solution is added that reacts with the enzyme-antibody-target
complex to produce measurable signal. The intensity of this signal is
directly proportional to the concentration of target present in the
original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 9.8%
- Intraassay CV: 5.3%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
There are no specifications
There are no report
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