Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

The ViraPower™ HiPerform™ T-REx™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3⁄TO⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway® technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Features

• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Contents

• 6 ug of pLenti6.3⁄TO⁄V5-DEST™ vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
• 20 ug of pLenti3.3⁄TR vector (40 µL of vector at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 10 ug of pLenti6.3⁄TO⁄V5-GW⁄lacZ plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 1 ml of Tetracycline (10 mg⁄mL in water)
• One Shot™ Stbl3™ Chemically Competent E. coli (C737303): 21 X 50 µL of Stbl3™ Cells
• 6 mL of S.O.C. Medium
• 50 µL of pUC19 control DNA (10 pg⁄µL).
• pENTR™ Gus positive control plasmid.