Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
On demand
Order number :
A11144
Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
The ViraPower™ HiPerform™ T-REx™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3⁄TO⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
- Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
- Easy recombination-based cloning using Gateway® technology
- Stable, long-term, tetracycline-regulated expression
- Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems
Features
- WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
- Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
- Blasticidin selection marker for stable selection under control of SV40 promoter
Contents
- 6 ug of pLenti6.3⁄TO⁄V5-DEST™ vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
- 20 ug of pLenti3.3⁄TR vector (40 µL of vector at 0.5 µg⁄µL in TE Buffer, pH 8.0)
- 10 ug of pLenti6.3⁄TO⁄V5-GW⁄lacZ plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
- 1 ml of Tetracycline (10 mg⁄mL in water)
- One Shot™ Stbl3™ Chemically Competent E. coli (C737303): 21 X 50 µL of Stbl3™ Cells
- 6 mL of S.O.C. Medium
- 50 µL of pUC19 control DNA (10 pg⁄µL).
- pENTR™ Gus positive control plasmid.
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