Invitrogen™ ChargeSwitch™ gDNA Rendered Meat Purification Kit

Invitrogen™ ChargeSwitch™ gDNA Rendered Meat Purification Kit

The ChargeSwitch® gDNA Rendered Meat Purification Kit allows for rapid and efficient purification of genomic DNA (gDNA) from animal feed, animal meal, and heparin products for downstream molecular analysis. The kit is listed in the U.S. Food and Drug Administration Center for Veterinary Medicine’s protocols as a sample preparation method suitable for monitoring crude heparin quality and testing animal feed for banned materials such as specific animal proteins.
The ChargeSwitch® gDNA Rendered Meat Purification Kit protocol starts with prepared lysates and takes approximately 15 minutes to complete without centrifugation, vacuum, or organic solvents (for batches of up to 5 samples per operator—larger batch sizes will require longer processing times). The kit employs a magnetic bead with a switchable surface. Low pH conditions cause binding of DNA to the bead while high pH conditions cause the release of the DNA.
Note: The number of reactions possible with this kit is dependent upon the starting sample type: animal feed & meal samples = 50 preparations, heparin products = 25 preparations.

Features

• Not High-throughput Compatible (Manual)
• Purification Target: Genomic DNA
• Sample Type: Biopharmaceutical Production Samples, Food & Environmental Samples
• Green Features: Fewer resources used and less waste, Less hazardous

Contents

• Magnetic Beads: 1.1 mL
• SDS Solution: 10 mL 
• 10% Detergent: 10 mL 
• Lysis Buffer: 100 mL
• Precipitation Buffer: 38.5 mL
• Wash Buffer: 200 mL
• Elution Buffer: 15 mL