Invitrogen™ DNaseAlert™ QC System

Invitrogen™ DNaseAlert™ QC System

Ambion® DNaseAlert™ QC System is a patent pending technology provides the sensitive detection of DNases in a simple, easy-to-use fluorimetric assay that delivers results in real time. Contains sufficient reagents for 5 × 96 high throughput assays.
The DNaseAlert™ QC System contains a unique DNA substrate tagged with both a fluorescent reporter and a dark quencher. The uncleaved substrate exhibits minimal fluorescence due to the proximity of the fluor and the quencher. When DNases are present, the linkage between the fluor and quencher is severed, which leads to the emission of a bright orange signal upon excitation at the proper wavelength of light. The extent of cleavage of the substrate is proportional to the level of DNase contamination and can be monitored as an increase in fluorescence on a fluorometer.

Features

• Detect a range of different DNases in minutes
• Certify plastics, enzymes, solutions, and other biomaterials as "DNase-free" prior to DNase-sensitive applications such as PCR
• Combine with RNaseAlert® to quantitatively resolve both RNase and DNase activity simultaneously
  

Contents

• DNaseAlert Substrate (2 nmol/tube): 5 tubes 
• 10X NucleaseAlert Buffer: 11 mL 
• DNase I (2 U/μL): 20 μL 
• TE Buffer (pH 7.0): 6 mL 
• RNaseZap® Solution: 250 mL
• Non-stick Tubes: 50 
• Nuclease-free Water: 50 mL