Invitrogen™ MEGAscript™ RNAi Kit with Manual

Invitrogen™ MEGAscript™ RNAi Kit with Manual

The Ambion® MEGAscript® RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian systems. The MEGAscript RNAi Kit uses Ambion's patented high-yield transcription technology and includes reagents for transcription, nuclease digestion, and clean up. The kit includes sufficient reagents for 20 reactions.

Synthesize Large Amounts of dsRNA:
The ability to generate large amounts of clean, double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit allows RNAi researchers to synthesize 50 to 100 µg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10–50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR-generated DNA template containing T7 RNA Polymerase promoters on both ends of the template, eliminating the need for a separate annealing step.

Features

• Control Type: Transcription Control
• Sample Type: Double-Stranded RNA (Long)

Contents

• T7 Enzyme Mix: 40 μL
• 10X T7 Reaction Buffer: 40 μL
• ATP Solution: 40 μL
• CTP Solution: 40 μL
• GTP Solution: 40 μL
• UTP Solution: 40 μL
• 10X Binding Buffer: 1 mL
• Elution Solution: 4 mL
• RNase: 40 μL
• DNase I: 45 μL
• 10X Digestion Buffer: 100 μL
• 2X Wash Solution: 12 mL
• Control Template: 20 μL
• Nuclease-free Water: 10 mL
• Filter Cartridge: 20
• Collection Tubes: 20